Cyclin D1 anticorps

N° du produit ABIN302101

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin D1 dans WB, ELISA, IP, ICC, IHC (p), IHC (fro) et ICFC. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour Cyclin D1 anticorps (ABIN302101)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: CD1-1

Détail du produit anti-Cyclin D1 anticorps

Fonction: Anti-Cyclin D1 Purified

Specificité: The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Réactivité croisée (Details): Human, Rat, Other not determined

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Purified cyclin D1 protein

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/mL, positive tissue: colon.
Western blotting: Recommended dilution: 1 μg/mL.
Immunocytochemistry: Recommended dilution: 1 μg/mL.
Immunoprecipitation: Recommended dilution: 1 μg/mL.
Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0.
Flow cytometry: Recommended dilution: 4-10 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Autre désignation: Cyclin D1

Sujet: Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

ID gène: 595

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling